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2x hafm wnt3a conditioned media in house  (InvivoGen)


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    Structured Review

    InvivoGen 2x hafm wnt3a conditioned media in house
    2x Hafm Wnt3a Conditioned Media In House, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 3029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2x+hafm+wnt3a+conditioned+media+in+house/Primocin/pmc13088643-95-40-34
    Average 99 stars, based on 3029 article reviews
    2x hafm wnt3a conditioned media in house - by Bioz Stars, 2026-10
    99/100 stars

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    PDO:

    Article Title: Spatially-resolved subtype progression reveals metabolic vulnerabilities in pancreatic ductal adenocarcinoma
    Article Snippet: .. Growth medium for PDO culture was prepared by combining 19 mL of organoid splitting medium Advanced DMEM/F12 (Gibco, 11320033) supplemented with 1x GlutaMAX (Gibco, 35050061), 1x HEPES (Gibco, 15630106), and 1 mL Primocin (1x, InvivoGen, ant-pm-05) with 25 mL of 2x hAFM Wnt3a-conditioned media (in-house), 5 mL of 10x R-spondin-conditioned media (in-house), 50 μL mNoggin (100 μg/mL, Peprotech, 25038100UG), 25 μL A83-01 (1 mM, Tocris, 2939), 50 μL Human Epidermal Growth Factor (hEGF; 500 μg/mL, Invitrogen, PIRP8661), 50 μL human Fibroblast Growth Factor-10 (hFGF-10; 100 mg/mL, Peprotech, 10026100UG), 50 μL Gastrin I (100 μM, Sigma, 10047-33-3), 125 μL N-acetylcysteine (500 mM, Sigma, 616-91-1), 500 μL Nicotinamide (1 M, Sigma, 98-92-0), 1 mL B-27 supplement (50x, Gibco, 17504001), and 100 μL Primocin (50 mg/mL, InvivoGen, ant-pm-05). .. PDAC organoids were generated by seeding 4,000 cells per well into 96-well plates pre-coated with 50 μL of Matrigel (Corning).



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    InvivoGen 2x hafm wnt3a conditioned media in house
    2x Hafm Wnt3a Conditioned Media In House, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2x+hafm+wnt3a+conditioned+media+in+house/Primocin/pmc13088643-95-40-34
    Average 99 stars, based on 1 article reviews
    2x hafm wnt3a conditioned media in house - by Bioz Stars, 2026-10
    99/100 stars
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